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Ion exchange chromatography of nucleotides
Summary
Type:
GPC
Application #:
107200
Catalog#:
719469 - VA 50/4.6 Nucleogel SAX 1000-8
Detection:
a) UV, 280nm b) 260nm
Conditions:
a) eluent: 0.01M NaH2PO4 pH 2.58, flow rate 1ml/min b) eluent A: 0.02 M KH2PO4 pH 2.6, eluent B: 0.5M KH2PO4 pH 3.5, linear gradient 0 - 100% B in 20 min, flow rate 1ml/min
Authors:
MACHEREY-NAGEL
Source:
LC department
Details
Legend: a) Isocratic elution of 2',3'-monophosphate nucleotides in 20 minutes, 1,2: CMP, 3,4: AMP, 5,6: UMP, 7,8: GMP. b) Separation of mono-, di- and triphosphate nucleotides, 1. CMP, 2. AMP, 3. UMP, 4.
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